And if all you care about is that the message is knocked down, then a western blot looking at protein expression might also suffice. However, after making a mutation, do you know if all of the cells contain the same mutation with the same expression profiles, and are therefore homogenous? If you have 100% transfection efficiency using a GFP reporter, for example, then at least you know that all of your cells were targeted for the mutation. Making mutations in mammalian cell lines is becoming much easier, especially with advanced molecular engineering techniques such as CRISPR/Cas9, among others.
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